Compound name
CHIR99021
Protein family
Protein Kinase
Target name
GSK3A
Affinity biochemical (nM)
10
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000127b
SMILES
Cc1c[nH]c(-c2cnc(NCCNc3ccc(C#N)cn3)nc2-c2ccc(Cl)cc2Cl)n1
InChIKey
AQGNHMOJWBZFQQ-UHFFFAOYSA-N
NCBI gene ID
2931
UniProt ID
P49840
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Enzymatic assay (phosphorylation of peptide substrate)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/12606497/
Affinity biochemical relation
=
Selectivity platform
KinomeScan (Carna Biosciences)
Selectivity platform number of targets
402
Selectivity remarks
Screened at 10 µM, closest targets as % of control: CDKL2(3.8%), PLK1 (5.4%), PIK3CG (14%), TAOK1 (12%), TAOK2 (13%), full screening data available as supporting information, https://pubs.acs.org/doi/10.1021/acs.jmedchem.8b01040#; In-vitro potency (enzymatic assay, 22 kinases), all targets >5 µM, CDC2, ERK2, PRKCA, PRKCE, AKT1, RPS6KB1, RSK2, SRC, TEK, FLT1, KDR,bFGFRTK, IGF1RTK, INSR, AMPK, PDK1, CHEK1, CSNK1E, DNAPK, PIP3K; >800-fold selectivity against 23 additional enzymes and 22 receptors, https://pubmed.ncbi.nlm.nih.gov/12606497/; Screened at 1 µM (against 71 kinases, in vitro), closest targets as % of control PLK1 (41%), MELK(45%), CDK2/cyclinA(53%), full screening data available in paper, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2267365/;
Selectivity Source Knowledge
https://pubs.acs.org/doi/full/10.1021/acschembio.6b00306
Selectivity Number of Off-targets
2