Chemical probes are cell-active, selective, highly validated research tools that can be used to decipher the biology of their target. They are useful assets for example, in phenotypic assays, or as a starting point for medicinal chemistry campaigns. Chemical probes developed by the EUbOPEN consortium are peer reviewed by an external committee and available upon request. The probes are released with a structurally similar inactive negative control compound (where feasible) and fulfill the following criteria:
| Potency | ≤ 100 nM in a biochemical or biophysical assay |
| Selectivity |
≥ 30-fold over related proteins |
| Evidence of target engagement in cells | ≤ 1 µM for the druggable target or 10 µM for shallow PPI targets |
| Cytotoxicity | ≥ 10 µM, unless target mediated |
For more information, or to request a probe, click on the probe link below.
| Probe name | Target | Negative control | Web link | Probe package |
|---|---|---|---|---|
| UNC8732 | NSD2 | UNC8884 | UNC8732: A chemical probe (degrader) for NSD2 | EUbOPEN_ProbeSheet_UNC8732_v1.pdf203.76 KB |
| PFI-8 | YEATS family | PFI-8N | PFI-8: A pan chemical probe for the YEATS family | EUbOPEN_ProbeSheet_PFI-8_v1.pdf187.15 KB |
| OICR11029 | BCL6 | OICR11600 | OICR11029: A chemical probe for BCL6 | EUbOPEN_ProbeSheet_OICR11029_v1.pdf199.94 KB |
| BI-5524 | ELANE (Elastase) | BI-5525 | BI-5524: A Chemical Probe for ELANE | EUbOPEN_ProbeSheet_BI_5524_v1.pdf213.1 KB |
| WEB2086 | PTAFR (Platelet activating factor receptor) | WEB2387 | WEB2086: A Chemical Probe for PTAFR | EUbOPEN_ProbeSheet_WEB2086_v1.pdf223.34 KB |
| BIBO3304 | NPY1R (Neuropeptide Y Receptor Y1) | BIBO3457 | BIBO3304: A Chemical Probe for NPY1R | EUbOPEN_ProbeSheet_BIBO3304_v1.pdf227.56 KB |
| SGK3-PROTAC1 | SGK3 (Serum/Glucocorticoid Regulated Kinase Family Member 3) | cisSGK3-PROTAC1 | SGK3-PROTAC1: A Chemical Probe for SGK3 | EUbOPEN_ProbeSheet_SGK3_PROTAC1_v1.pdf215.9 KB |
| VZ185 | BRD7 and BRD9 | cisVZ185 | VZ185: A Chemical Probe for BRD7 and BRD9 | EUbOPEN_ProbeSheet_VZ185_v1.pdf230.75 KB |
| BAY-277 | METAP2 (Methionyl Aminopeptidase 2) | BAY-8805 | BAY-277: A Chemical Probe for METAP2 | EUbOPEN_ProbeSheet_BAY_277_v1.pdf207.35 KB |
| BAY-6096 | ADRA2B (Adrenoceptor Alpha 2B) | BAY-726 | BAY-6096: A Chemical Probe for ADRA2B | EUbOPEN_ProbeSheet_BAY_6096_v1.pdf219.39 KB |
| BAY 1753011 | AVPR1A (Arginine Vasopressin Receptor 1A), AVPR2 (Arginine Vasopressin Receptor 2) | BAY-2297 | BAY 1753011: A Dual Probe for AVPR1A and AVPR2 | EUbOPEN_ProbeSheet_BAY 1753011_v1.pdf233.22 KB |
| JA310 | MST3 (Mammalian STE20-like protein kinase 3)/ STK24 (Serine/threonine-protein kinase 24) MST4 (Mammalian STE20-like protein kinase 4)/ STK26 (Serine/threonine-protein kinase 26) | JA262 | JA310: A Chemical Probe for MST3/4 | EUbOPEN_ProbeSheet_JA310_v1.pdf208.28 KB |
| BI-3812 (inhibitor) | BCL6 (BCL6 transcription repressor, Zinc Finger Protein 51) | BI-5273 | BI-3812: A Chemical Probe for BCL6 | EUbOPEN_ProbeSheet_BI_3812_v1.pdf236.36 KB |
| BI-3802 (degrader) | BCL6 (BCL6 transcription repressor, Zinc Finger Protein 51) | BI-5273 | BI-3802: A Chemical Probe for BCL6 | EUbOPEN_ProbeSheet_BI_3802_v1.pdf234.29 KB |
| JP3000 | RXRA, RXAB, RXRG | JP3001 | JP3000: A Chemical Probe for RXR | EUbOPEN_ProbeSheet_JP3000_v1.pdf179.46 KB |
| S213726 | Kelch-like ECH-associated protein 1, KEAP1 (KLHL19) | S214489 | EUbOPEN_ProbeSheet_S213726_v1.pdf219.41 KB | |
| SGC-GSK3-1 | GSK3A, GSK3B | SGC-CDKL5/GSK3-1N | SGC-GSK3-1: A Chemical Probe for GSK3⍺ and GSK3β | EUbOPEN_ProbeSheet_GSK3_v1.pdf262.03 KB |
| SGC-CDKL5/GSK3-1 | STK9, GSK3A, GSK3B | SGC-CDKL5/GSK3-1N | SGC-CDKL5/GSK3-1: A Chemical Probe for CDKL5, GSK3⍺, and GSK3β | EUbOPEN_ProbeSheet_CDKL5GSK3_v1.pdf288.71 KB |
| SGC-CAF382-1 | STK9, PITALRE, PCTAIRE, PCTK1, PCTAIRE2, PCTK2, PCTAIRE3, PCTK3 | SGC-CAF268-1N | SGC-CAF382-1: A Chemical Probe for CDKL5, CDK9, CDK16, CDK17, and CDK18 | EUbOPEN_ProbeSheet_CAF382_v1.pdf293.65 KB |
| BAY-7081 | PDE9A (Phosphodiesterase 9A) | BAY-7424 | BAY-7081: A Chemical Probe for PDE9A | EUbOPEN_ProbeSheet_BAY_7081_v1.pdf149.05 KB |