Compound name
Alisertib
Protein family
Protein Kinase
Target name
STK6
Affinity biochemical (nM)
1.2
Affinity on-target cellular (nM)
6.7
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0000133b
SMILES
COC1=C(C(=CC=C1)F)C2=NCC3=CN=C(N=C3C4=C2C=C(C=C4)Cl)NC5=CC(=C(C=C5)C(=O)O)OC
InChIKey
ZLHFILGSQDJULK-UHFFFAOYSA-N
NCBI gene ID
6790
UniProt ID
O14965
Synonyms
BTAK, AurA, STK7, ARK1, PPP1R47, AIK
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Radioactive Flashplate assay
Affinity Biochemical Source Knowledge
https://aacrjournals.org/clincancerres/article/17/24/7614/204920/Characterization-of-Alisertib-MLN8237-an
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Immunostaining (pThr288 autophosphorylation assay in HeLa cells)
Affinity on-target cellular source knowledge
https://aacrjournals.org/clincancerres/article/17/24/7614/204920/Characterization-of-Alisertib-MLN8237-an
Affinity on-target cellular relation
=
Selectivity platform
SelectScreen kinase panel (Invitrogen)
Selectivity platform number of targets
205
Selectivity remarks
Screened at 1 µM, closest targets as % of inhibition: BLK (92%), CAMK2A (95%), EPHA2 (100%), EPHA4 (94%), FGFR2 (90%), FGR (97%), MAPKAPK3 (91%), NTRK3 (91%); Proteomics (Kinobeads assays), in-vitro potencies of closest targets: Kdapp(AURKA) = 5 nM, Kdapp(ACAD10) = 78 nM, Kdapp(AURKB) = 195 nM, Kdapp(PDPK1) = 289 nM, Kdapp(EPHA2) = 309 nM, Kdapp(EPHA4) = 455 nM, Kdapp(DNAJA1) = 962 nM, Kd(ABL2) = 1009 nM, Kdapp(GNAI2) = 184 nM, Kdapp(PSMA7) = 405 nM, Kdapp(MCM4) = 629 nM, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6542668/;
Selectivity Source Knowledge
https://aacrjournals.org/clincancerres/article/17/24/7614/204920/Characterization-of-Alisertib-MLN8237-an