Compound name
GSK2801
Protein family
Bromodomain
Target name
BAZ2A@BRD
Affinity biochemical (nM)
400
Affinity on-target cellular (nM)
3981
CG-Set
Epigenetic set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000222b
SMILES
CCCOc1ccn2c(C(C)=O)cc(-c3ccccc3S(C)(=O)=O)c2c1
InChIKey
KHWCPNJRJCNVRI-UHFFFAOYSA-N
NCBI gene ID
11176
UniProt ID
Q9UIF9
Synonyms
KIAA0314, TIP5, WALp3
Mode of action
Inhibitor
Negative control
GSK8573
Affinity biochemical definition
IC50
Affinity biochemical assay type
AlphaScreen assay
Affinity Biochemical Source Knowledge
https://doi.org/10.1021/acs.jmedchem.5b00209
Affinity biochemical relation
=
Affinity on-target cellular definition
Kd
Affinity on-target cellular assay type
Chemoproteomic competition binding assay (endogenous full-length BAZ2A from HuT78 extracts)
Affinity on-target cellular relation
=
Selectivity platform
Bromodomain panel, literature
Selectivity platform number of targets
46
Selectivity remarks
Screened at 10 µM in DSF assays, dTm(BAZ2A) = 4.1 K, dTm(BAZ2B) = 2.7 K, closest targets: dTm(TAF1L) = 3.4 K, dTm(BRD9) = 2.3 K; In vitro follow up (biolayer interferometry experiment) at 0.2 µM: Kd(BAZ2B) = 0.1 µM, Kd(BRD9) = 1.2 µM, Kd(TAF1L) = 3.19 µM, (ITC experiment): Kd(TAF1L) = 3.2 µM, Kd(BRD9) = 1.1 µM; Screened in CEREP panel against 55 receptors and ion channels at 10 µM, closest target as % of contr.: Melatonin (MT-1) receptor(4%)
Selectivity Source Knowledge
https://doi.org/10.1021/acs.jmedchem.5b00209
https://www.thesgc.org/chemical-probes/GSK2801
Selectivity Number of Off-targets
0