Compound name
NVS-BPTF-1
Protein family
Bromodomain
Target name
BPTF@BRD
Affinity biochemical (nM)
71
Affinity on-target cellular (nM)
16
CG-Set
Epigenetic set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000253b
SMILES
Cc1c(Nc2ccc(S(=O)(=O)N3CCN(C)CC3)cc2F)nc2ccc(-c3cnn(C4CC4)c3)cn2c1=O
InChIKey
JYTISQGEFSHUIR-UHFFFAOYSA-N
NCBI gene ID
2186
UniProt ID
Q12830
Synonyms
FAC1, NURF301
Mode of action
Inhibitor
Negative control
NVS-BPTF-C
Affinity biochemical definition
Kd
Affinity biochemical assay type
BioLayer Interferometry assay (BLI)
Affinity Biochemical Source Knowledge
https://doi.org/10.1016/j.bmcl.2021.128208
https://www.thesgc.org/chemical-probes/NVS-BPTF-1
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (HEK293T cells)
Affinity on-target cellular relation
=
Selectivity platform
BROMOscan
Selectivity platform number of targets
32
Selectivity remarks
Screened at 1 µM, closest targets: Kd(CERC2) = 66 nM, Kd(GCN5L2) = 62 nM, Kd(PCAF) = 74 nM; Follow-up IC50s in cellular NanoBRET assay: IC50(CERC2/GCN5L2/PCAF) >10 µM; DSF screen against 48 human bromodomains, dTm(BPTF) = 6.16 K, closest targets: dTm(CERC2) = 2.48 K, dTm(BRD7) = 1.95 K, dTM(PCAF) = 1.53 K; Screened in NIBR principial panel against 12 GPCRs, 3 nuclear receptors, 3 transporters and 7 other enzymes, closest tagets: IC50(Ad3) = 4.9 µM, IC50(D3) = 3.5 µM, IC50(H3) = 4 µM, others >30 µM, Screened in NIBR kinase panel against 48 kinases, no target with IC50 <30 µM;
Selectivity Source Knowledge
https://www.thesgc.org/chemical-probes/NVS-BPTF-1
Selectivity Number of Off-targets
3