Compound name
ABT-100
Protein family
Transferase
Target name
FNTB
Affinity biochemical (nM)
0.05
Affinity on-target cellular (nM)
0.73
CG-Set
Other targets
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000289c
SMILES
CCCCN(CCCC)C(=O)CN1C[C@@H](c2cc(OC)c3c(c2)OCO3)[C@H](C(=O)O)[C@H]1CC(C)(C)CCC
InChIKey
OAEWNSKRLBVVBV-OBTVHEKISA-N
NCBI gene ID
2342
UniProt ID
P49356
Synonyms
FNTB
Mode of action
Inhibitor
Negative control
A-108
Affinity biochemical definition
IC50
Affinity biochemical assay type
Scintillation proximity assay (SPA)
Affinity Biochemical Source Knowledge
https://doi.org/10.1097/00001813-200511000-00004
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/ABT-100
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Western Blot (inhibition of Ras in NIH 3T3 H-Ras cells)
Affinity on-target cellular relation
=
Selectivity platform
CEREP panel (Eurofins)
Selectivity platform number of targets
75
Selectivity remarks
Screened at 3 µM against 75 ion channels, receptors, GPCRs and enzymes (ligand binding assay), closest target as % inhibition: PRCP (97%), >100 000-fold selective for FNTB over the closely related enzyme PGGT1B (GGTase I); Screened against 468 kinases (KinomeScan DiscoverX), at 1 µM, closest targets as % of contr.: EPHA1 (38%), NEK7 (46%), BRSK2 (48%), GRK3 (48%), follow-up Kd values: IC50(EPHA1/NEK7/BRSK2/GRK3) >10 µM; Screened against 45 GPCR targets (PDSP screen) at 10 µM, closest target: Ki(HRH1) >10 µM;
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/ABT-100
Selectivity Number of Off-targets
0