Compound name
BAY-474
Protein family
Protein Kinase
Target name
MET
Affinity biochemical (nM)
1
Affinity on-target cellular (nM)
2.9
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000293b
SMILES
CC1=C(C#N)C(c2ccc3[nH]nc(C)c3c2)C(C#N)=C(C)N1
InChIKey
QKVFMAAIXZONRN-UHFFFAOYSA-N
NCBI gene ID
4233
UniProt ID
P08581
Synonyms
HGFR, RCCP2, DFNB97
Mode of action
Inhibitor
Negative control
BAY-827
Affinity biochemical definition
IC50
Affinity biochemical assay type
Homogeneous Time Resolved Fluorescence Assay
Affinity Biochemical Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BAY-474
Affinity biochemical relation
<
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Cellular mechanistic assay (phosphorylation of MET in NKM-45 cells)
Affinity on-target cellular relation
=
Selectivity platform
Millipore panel
Selectivity platform number of targets
218
Selectivity remarks
Screened at 1 µM, closest target: IC50(RPS6KA3) = 0.9 µM, >1000 fold selective against all other kinases; Eurofins-Panlabs radioligand binding assay (screened against 68 targets, at 10 µM): clean profile; Screened against 45 GPCR targets (PDSP screen) at 10 µM: clean profile
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BAY-474
Selectivity Number of Off-targets
0