Compound name
BTZO-1
Protein family
Tautomerase
Target name
MIF
Affinity biochemical (nM)
68.6
CG-Set
Other targets
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000301b
SMILES
O=c1nc(-c2ccccn2)sc2ccccc12
InChIKey
GBAKVEWPYUIGHN-UHFFFAOYSA-N
NCBI gene ID
4282
UniProt ID
P14174
Synonyms
MIF, GLIF, MMIF
Mode of action
Activator
Negative control
BTZO-4
Affinity biochemical definition
Kd
Affinity biochemical assay type
Scintillation proximity assay (SPA; measurement of selective [83H]-BTZO-1 binding to human MIF-His)
Affinity Biochemical Source Knowledge
https://doi.org/10.1016/j.chembiol.2010.10.011
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BTZO-1
Affinity biochemical relation
=
Selectivity remarks
Screened against 468 kinases (KinomeScan DiscoverX) at 1 µM, closest targets as % of contr.: CDK8 (36%), EIF2AK4 (47%); Screened against 45 GPCR targets (PDSP screen) at 10 µM, closest target: Ki(GABAA/BZP) = 934 nM; Selectivity against recently discovered D-Dopachrome Tautomerase (DDT) is unknown.
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BTZO-1