Compound name
BI-2545
Protein family
Hydrolase
Target name
ENPP2
Affinity biochemical (nM)
2.2
Affinity on-target cellular (nM)
29
CG-Set
Other targets
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000318b
SMILES
O=C(NC[C@H]1[C@@H]2CN(C(=O)OCc3cc(C(F)(F)F)cc(C(F)(F)F)c3)C[C@H]12)c1ccc2nn[nH]c2c1
InChIKey
ZDOBSAPHUUUOHX-OSYLJGHBSA-N
NCBI gene ID
5168
UniProt ID
Q13822
Synonyms
ENPP2, ATX, PDNP2
Mode of action
Inhibitor
Negative control
BI-3017
Affinity biochemical definition
IC50
Affinity biochemical assay type
Biochemical assay (LPA measurement)
Affinity Biochemical Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BI-2545
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Inhibition of Autotaxin (whole blood assay)
Affinity on-target cellular source knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BI-2545
Affinity on-target cellular relation
=
Selectivity platform
KinomeScan (DiscoverX)
Selectivity platform number of targets
468
Selectivity remarks
Screened at 1 µM, closest targets as % of contr.: ASK (13%), NEK1 (44%); Screened at 10 µM against 45 GPCRs (PDSP screen), in-vitro potencies of closest targets: Ki(GABA/PBR) = 2.56 µM, Ki(SIGMAR1) =3.55 µM; Screened at 10 µM against 44 other enzymes (CEREP selectivity screen), closest targets as % inhibition: CACNA1C (80%), SCN1B (66%), SLC6A2 (61%), HTR2A (55%)
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/#!specificprobeoverview/BI-2545