Compound name
Borussertib
Protein family
Protein Kinase
Target name
AKT1
Affinity biochemical (nM)
0.8
Affinity on-target cellular (nM)
21
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000645a
SMILES
C=CC(=O)Nc1ccc2[nH]c(=O)n(C3CCN(Cc4ccc(-c5nc6cc[nH]c(=O)c6cc5-c5ccccc5)cc4)CC3)c2c1
InChIKey
HXBRBOYWXDLHDC-UHFFFAOYSA-N
NCBI gene ID
207
UniProt ID
P31749
Synonyms
RAC, PKB, PRKBA, AKT, RAC-alpha
Mode of action
Covalent-allosteric inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
HTFR assay
Affinity Biochemical Source Knowledge
https://doi.org/10.1002/anie.201909857
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (HEK293T cells)
Affinity on-target cellular relation
=
Selectivity platform
SelectScreen® Kinase Profiling (activity based Z’LYTE® assay)
Selectivity platform number of targets
100
Selectivity remarks
Closest target: AKT3 (83% of ctrl.); Follow up in vitro activity: IC50(AKT3) = 618 nM (HTFR assay, PMID: 31584233), IC50(AKT3) = 4.3 µM (NanoBRET assay)
Selectivity Source Knowledge
https://doi.org/10.1039/c8sc05212c
Selectivity Number of Off-targets
1