Compound name
640
Protein family
Protein Kinase
Target name
CAMK1
Affinity biochemical (nM)
1
Affinity on-target cellular (nM)
23
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0000878a
SMILES
CC(C)c1cc(Nc2nc(N3CCC[C@H](N)C3)ncc2C(N)=O)cc(C(C)C)n1
InChIKey
BWBUPDTUXQDHSX-AWEZNQCLSA-N
NCBI gene ID
8536
UniProt ID
Q14012
Synonyms
CaMKI, CaMKI-alpha
Mode of action
Inhibitor
Negative control
CS640s
Affinity biochemical definition
IC50
Affinity biochemical assay type
Enzymatic assay (Reaction Biology)
Affinity Biochemical Source Knowledge
https://pubs.acs.org/doi/10.1021/acs.jmedchem.9b01803
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (in HEK293T cells)
Affinity on-target cellular source knowledge
https://www.thesgc.org/chemical-probes/cs-640
Affinity on-target cellular relation
=
Selectivity platform
KinomeScan (DiscoverX)
Selectivity platform number of targets
450
Selectivity remarks
Screened at 1 µM, closest targets as % of control: PIP5K1C (0%), MEK5 (0.95%), RIPK4 (4.5%), MLK3 (99%); In-vitro potency of closest targets (enzymatic assay, Reaction Biology): IC50(PIP5K1C) = 11 µM, IC50(MEK5) = 0.025 µM, IC50(RIPK4) = 5.69 µM, IC50(MLK3) = 2.75 µM, https://pubs.acs.org/doi/10.1021/acs.jmedchem.9b01803#; Screened at 10 µM in Eurofins Cerep-Panlabs Safety Screen 44 (GPCRS, Transporters, Ion Channels, Nuclear Receptors, other enzymes), closest targets as % of inhibition or stimulation: dopamine transporter (100%), hERG channel (89%), norepinephrine transporter (87%), M1 (84%), M2(70%), M3(74%), acetylcholinesterase (84%), https://www.thesgc.org/chemical-probes/cs-640;
Selectivity Source Knowledge
https://pubs.acs.org/doi/10.1021/acs.jmedchem.9b01803