Compound name
SGC-CLK-1N
Protein family
Protein Kinase
Target name
CLK2
Affinity biochemical (nM)
10000
Affinity on-target cellular (nM)
10000
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001049a
SMILES
COC1=NN2N=CC(C3=NC(NC4=CC(C(F)(F)F)=CC(OC)=C4)=NC(C)=C3)=C2C=C1
InChIKey
BJVQXSHZMUFJBQ-UHFFFAOYSA-N
NCBI gene ID
1196
UniProt ID
P49760
Mode of action
Negative control for SGC-CLK-1 (UNC-CAF-170 )
Affinity biochemical definition
IC50
Affinity biochemical assay type
Enzymatic assay (Eurofins)
Affinity Biochemical Source Knowledge
https://www.thesgc.org/chemical-probes/SGC-CLK-1
Affinity biochemical relation
>
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (in HEK293T cells)
Affinity on-target cellular source knowledge
https://www.thesgc.org/chemical-probes/SGC-CLK-1
Affinity on-target cellular relation
>
Selectivity platform
KinomeScan (DiscoverX)
Selectivity platform number of targets
403
Selectivity remarks
Screened at 1 µM, no kinases with <45% of control; In-vitro potency (Eurofins Enzymatic assay): IC50(CLK3) >10 µM, IC50(DYRK1A) >10 µM, IC50(DYRK1B) >10 µM, IC50(DYRK2) >10 µM, IC50(DYRK3) >10 µM, IC50(HIPK1) >10 µM, IC50(HIPK2) >10 µM, IC50(HIPK3) >10µM, IC50(HIPK4) >10 µM, IC50(PIM2) >10 µM, https://www.thesgc.org/chemical-probes/SGC-CLK-1;
Selectivity Source Knowledge
https://www.thesgc.org/chemical-probes/SGC-CLK-1