Compound name
Methyltrienolone
Protein family
Nuclear Receptors
Target name
NR3C2
Affinity on-target cellular (nM)
0.5
CG-Set
Nuclear Receptor set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001073a
SMILES
C[C@@]1(CC[C@@H]2[C@@]1(C=CC3=C4CCC(=O)C=C4CC[C@@H]23)C)O
InChIKey
CCCIJQPRIXGQOE-XWSJACJDSA-N
NCBI gene ID
4306
UniProt ID
P08235
Synonyms
MR
Mode of action
Agonist
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
HEK293 Lysate Stable Transfection Competition binding assays (NR3C1, NR3C2, NR3C3), reporter gene assay (C2C12 cells were cotransfected with a ARE-TK-luciferase reporter and pCMV-hAR) (NR3C4)
Affinity on-target cellular source knowledge
https://doi.org/10.1021/acs.jmedchem.5b01168
Affinity on-target cellular relation
0
Selectivity platform
Nuclear receptor panel, bromodomain and kinase panel
Selectivity platform number of targets
23
Selectivity remarks
Screened at 1 µM againstNR1A1, NR1B1, NR1C3, NR1F3, NR1H3, NR1I1, NR1I2, NR1I3, NR2A1, NR2B1, NR4A1, NR5A2 and VP16 in a Gal4 hybrid reporter gene assay (HEK293T cells were cotransfected with pFR-Luc reporter, pRL-SV40 control reporter and pFA-CMV containing the Gal4-DBD fused with human NR-LBD of interest or pECE-SV40-Gal4-VP16): clean selectivty profile with no significant agonism/antagonism detected; Screened at 20 µM in a bromodomain and kinase panel against ABL1, AURKA, BRD4, BRPF1, CDK2, CSNK1D, FGFR3, GSK3B, MAPK1, TRIM24 in DSF assays: clean selectivity profile with no significant binding (?Tm < 2 K)