Compound name
GSK973
Protein family
Bromodomain
Target name
BRD2@BD2
Affinity biochemical (nM)
31.6
CG-Set
Epigenetic set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001113a
SMILES
[H][C@@]12COC[C@@]2([H])[C@@H]1NC(c1cc(C(NC)=O)c2c(c1)[C@H](c1ccccc1)[C@@H](CF)O2)=O
InChIKey
WZQLVEPIBAOOGF-RMMWZPCPSA-N
NCBI gene ID
6046
UniProt ID
P25440
Synonyms
KIAA9001, RING3, D6S113E, NAT, FSRG1
Mode of action
Inhibitor
Negative control
GSK943
Affinity biochemical definition
IC50
Affinity biochemical assay type
BET mutant TR-FRET assay (BD2)
Affinity Biochemical Source Knowledge
https://pubs.acs.org/doi/10.1021/acsmedchemlett.0c00247
Affinity biochemical relation
0
Selectivity platform
BROMOScan (DiscoverX)
Selectivity platform number of targets
40
Selectivity remarks
In-vitro potencies of closest targets in BROMOScan (DiscoverX): pKd(TAF1, BD2) = 6.4, pKd(TAF1L, BD2) = 5.7, pKd(BRD9) = 5.7, pKd(CECR2) = 5.5, pKd(BAZ2A) = 5.5, pKd(BAZ2B) = 5.1; Selective for the BD2 domain of BET proteins; Clean in selectivity screen against 48 targets (GPCRs, ion-channels, other targets); Chemoproteomics data (3 vectors) showed excellent non BCPs selectivity; Screened at 10 µM against 45 GPCRs (PDSP screen), in-vitro potencies of closest targets: Ki(DRD3) = 257.47 nM, Ki(DRD4) = 982.65 nM, Ki(DRD2) = 964.05 nM
Selectivity Source Knowledge
https://pubs.acs.org/doi/10.1021/acsmedchemlett.0c00247
Selectivity Number of Off-targets
0