Compound name
TX-85-1
Protein family
Protein Kinase
Target name
ERBB3
Affinity biochemical (nM)
23
Affinity on-target cellular (nM)
5000
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001283a
SMILES
C=CC(=O)Nc1cc(-c2nn(C3CCC(N4CCN(C(C)=O)CC4)CC3)c3ncnc(N)c23)ccc1Oc1ccccc1
InChIKey
FYICDSWKKFSYOM-UHFFFAOYSA-N
NCBI gene ID
2065
UniProt ID
P21860
Synonyms
HER3
Mode of action
Covalent inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
FRET binding assay
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/25326665/
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Western Blot assay (Her3 pull-down using TX1-85-1-biotin in PC9 GR4 Her3 cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/25326665/
Affinity on-target cellular relation
<
Selectivity platform
KiNativ® profiling
Selectivity platform number of targets
226
Selectivity remarks
Screened at 2 µM, closest targets as % of inhibition: HER3 (75.9%), LYN (79.8%), FYN (65.7%), HER2 (69.8%), full screening data are available as supporting information, https://pubmed.ncbi.nlm.nih.gov/25326665/;
In-vitro potency (Z'-LYTETM Kinase Assay, Invitrogen,): IC50(EGFR) = 5.6 µM, https://pubmed.ncbi.nlm.nih.gov/25326665/;
In-vitro potency (Z'-LYTETM Kinase Assay, Invitrogen,): IC50(HER2) = 2.63 µM, https://pubmed.ncbi.nlm.nih.gov/25326665/;
In-vitro potency (Z'-LYTETM Kinase Assay, Invitrogen,): IC50(EGFR) = 5.6 µM, https://pubmed.ncbi.nlm.nih.gov/25326665/;
In-vitro potency (Z'-LYTETM Kinase Assay, Invitrogen,): IC50(HER2) = 2.63 µM, https://pubmed.ncbi.nlm.nih.gov/25326665/;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/25326665/
Selectivity Number of Off-targets
1