Compound name
GNE-2256
Protein family
Protein Kinase
Target name
IRAK4
Affinity biochemical (nM)
1.4
Affinity on-target cellular (nM)
3
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001558a
SMILES
CC(C)(O)[C@H](F)CN1Cc2cc(NC(=O)c3cnn4cccnc34)c(N3CCOCC3)cc2C1=O
InChIKey
AUYCSWFYKYVCLD-HXUWFJFHSA-N
NCBI gene ID
51135
UniProt ID
Q9NWZ3
Synonyms
NY-REN-64
Mode of action
Inhibitor
Negative control
GNE-6689
Affinity biochemical definition
Ki
Affinity biochemical assay type
Biochemical assay
Affinity Biochemical Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!specificprobeoverview/GNE-2256
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
NanoBRET assay (HEK293T cells)
Affinity on-target cellular source knowledge
https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!specificprobeoverview/GNE-2256
Affinity on-target cellular relation
=
Selectivity platform
SelectScreen Kinase Profiling (Thermo Fisher Scientific)
Selectivity platform number of targets
221
Selectivity remarks
Screened at 1 µM, closest targets as % of inhibition: FLT3 (90.8%), MAP4K4 (86.3%), MINK1 (80.5%), NTRK1 (71%), NTRK2 (86%), JAK1 (83.5%), JAK2 (80.5%), LRRK2 (91.4%), TAK-TAB (74.5%); In-vitro potency of closest targets (Z'-Lyte kinase biochemical assay, conc. ATP = Km(app), Thermo Fisher Scientific): IC50(FLT3) = 177 nM, IC50(MAP4K4) = 680 nM, IC50(MINK1) = 879 nM, IC50(NTRK1) = 313 nM, IC50(NTRK2) = 259 nM, IC50(JAK1) = 282 nM, IC50(JAK2) = 486 nM, IC50(LRRK2) = 198 nM, IC50(TAK-TAB) = 966 nM (https://pubs.acs.org/doi/10.1021/acsmedchemlett.9b00380); In-cellulo follow-up of closest targets (NanoBRET assay, HEK293T cells): EC50(LRRK2) = 5.2 µM, EC50(NTRK2) = 5.6 µM, EC50(NTRK1) = 11 µM, EC50(FLT3) >50 µM, EC50(JAK1) >50 µM, EC50(JAK2) >50 µM (https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!specificprobeoverview/GNE-2256);
Selectivity Source Knowledge
https://pubs.acs.org/doi/10.1021/acsmedchemlett.9b00380