Compound name
JNJ-54119936
Protein family
Nuclear Receptor
Target name
RORC
Affinity biochemical (nM)
5.3
Affinity on-target cellular (nM)
30
CG-Set
NR set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001568a
SMILES
COc1nc2ccc([C@@](O)(c3ccccc3)C3CCN(C(C)=O)CC3)cc2c(Cl)c1Cc1ccc(-n2cccn2)cc1
InChIKey
QBIGUDRHHJTXKG-UUWRZZSWSA-N
NCBI gene ID
6097
UniProt ID
P51449
Synonyms
RZRG, RORG, NR1F3, TOR
Mode of action
Inverse agonist
Negative control
JNJ-53721590
Affinity biochemical definition
qKd
Affinity biochemical assay type
ThermoFlour binding assay (RORC)
Affinity Biochemical Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!detailedpotencydataview/JNJ-54119936
Affinity biochemical relation
0
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
One-hybrid reporter assay (HEK293T cells co-transfected with pFR-Luc reporter and pRL-CMV reporter, pCMV-BD containing the GAL4 DNA-binding domain fused with full-length human RORγt using 6 µM compound)
Affinity on-target cellular source knowledge
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155022/
Affinity on-target cellular relation
0
Selectivity platform
Nuclear receptor panel (in-house data Janssen)
Selectivity platform number of targets
15
Selectivity remarks
Screened at 3 µM against PPARa/d/g, LXRa/b, CAR, FXR, GR, Era/b, Tra/b, VDR, PXR, RXRa: clean selectivity profile with no significant agonism/antagonism detected; Screened at 1 µM and 10 µM in CEREP panel against 50 receptors and other enzymes: clean selectivity profile with no target inhibited >50%; Screened at 10 µM against 50 kinases (DiscoverX), clean selectivity profile with no target inhibited >50%; Screened at 10 µM against 45 GPCRs (PDSP screen), in-vitro potencies of closest targets: Ki(TMEM97) = 636.99 nM, Ki(SIGMAR1) = 3125.58 nM, Ki(ADRA2B) = 9223.59 nM
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!specificprobeoverview/JNJ-54119936
Selectivity Number of Off-targets
0