Compound name
8RK64
Target name
UCHL1@Protease
Affinity biochemical (nM)
320
Affinity on-target cellular (nM)
3000
CG-Set
Other targets set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001574a
SMILES
N#CN1CC[C@H](C(=O)Nc2nc3c(s2)CN(C(=O)CN=[N+]=[N-])CC3)C1
InChIKey
KIWKRCCIHSGWQS-VIFPVBQESA-N
NCBI gene ID
7345
UniProt ID
P09936
Synonyms
HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1
Mode of action
covalent inhibitor
Negative control
JYQ88
Affinity biochemical definition
IC50
Affinity biochemical assay type
Biochemical activity assay using a fluorogenic Ub-Rho-morpholine (inhibition of UCHL1 using 2 mM cysteine)
Affinity Biochemical Source Knowledge
https://pubs.acs.org/doi/10.1021/jacs.0c07726
Affinity biochemical relation
0
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Activity-based protein profiling assay (ABPP experiment, inhibition of UCHL1 in HEK293 cells)
Affinity on-target cellular source knowledge
https://pubs.acs.org/doi/10.1021/jacs.0c07726
Affinity on-target cellular relation
<
Selectivity platform
GPCR panel (PDSD screen)
Selectivity platform number of targets
45
Selectivity remarks
Screened at 10 µM, in-vitro potency of closest target: Ki(SIGMAR1) = 3.429 µM; No inhibition of other deubiquitylating enzymes in activity-based protein profiling assay (ABPP) experiment; Selectivity outside target family (2‐step labeling Pull‐down proteomics experiment): off-target PARK7 (has cysteine in active site) identified
Selectivity Source Knowledge
https://www.sgc-ffm.uni-frankfurt.de/chemProbes#!gpcrscandataview/8RK64
Selectivity Number of Off-targets
0