Compound name
MSC2530818
Protein family
Protein Kinase
Target name
CDK8
Affinity biochemical (nM)
2.6
Affinity on-target cellular (nM)
6.5
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0001623a
SMILES
Cc1n[nH]c2ncc(C(=O)N3CCC[C@H]3c3ccc(Cl)cc3)cc12
InChIKey
ODRITQGYYWHQGM-INIZCTEOSA-N
NCBI gene ID
1024
UniProt ID
P49336
Synonyms
K35
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
FRET-based Lanthascreen binding competition assay
Affinity Biochemical Source Knowledge
https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b00597#
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
7dF3 WNT-specific reporter gene assay
Affinity on-target cellular source knowledge
https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b00597#
Affinity on-target cellular relation
=
Selectivity platform
Kinase panel (literature)
Selectivity platform number of targets
264
Selectivity remarks
Screened at 1 µM, closest targets as % of inhibition: P70S6K (47%), SGK (39%), CDK1 (cyclinB, 2%), CDK2 (cyclinA, -7%), CDK2 (cyclinE, 18%), CDK3 (cyclinE, 2%), CDK5(p25, -1%), CDK5 (p35, -1%), CDK6 (cyclinD3, 12%), CDK7 (cyclinH/MAT1, 7%), CDK9 (cyclinT1, 13%); In-vitro potency of closest targets (enzymatic assays): IC50(GSK3A) = 691 nM, IC50(FLT3) >30 µM, IC50(NTRK3) >30 µM; Screened at 10 µM against 59 receptors and ion channels (CEREP panel, binding assay), closest targets as % of inhibition: A1 (48%), D2 (54%); In-vitro potency of closest targets (CEREP binding assay): IC50(D2) = 8.5 µM, https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b00597#;
Selectivity Source Knowledge
https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b00597#