Compound name
BI-0900
Protein family
RNA Polymerase
Target name
HCV NS5B
Affinity biochemical (nM)
6400
Affinity on-target cellular (nM)
10000
CG-Set
Other targets set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001703a
SMILES
CC(C)N(C(=O)C1CCOCC1)c1cc(F)c(Oc2ncc(Cn3nccn3)cc2C(F)(F)F)cc1C(=O)O
InChIKey
MXZMIFJCRSXQNY-UHFFFAOYSA-N
Mode of action
Negative control for BI-0588
Affinity biochemical definition
IC50
Affinity biochemical assay type
Scintillation proximity HCV polymerase assay (measures the incorporation of 3H-UTP during the elongation of RNA primer with recombinant protein His-NS5Bd21)
Affinity Biochemical Source Knowledge
https://www.opnme.com/molecules/hcv-polymerase-bi-0588
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Cell-based luciferase reporter HCV RNA replication assay (MP-1 cells with 10% FBS, 28 h incubation)
Affinity on-target cellular source knowledge
https://www.opnme.com/molecules/hcv-polymerase-bi-0588
Affinity on-target cellular relation
>
Selectivity platform
SafetyScreen44™ (Eurofins)
Selectivity platform number of targets
44
Selectivity remarks
Screened at 10 µM against a panel of ion channels, GPCRs, hydrolases, and other enzymes, closest target as % of contr.: DELTA2 (33%), full screening data available on OpenMe website, https://www.opnme.com/search-results/BI-9307;
Selectivity Source Knowledge
https://www.opnme.com/search-results/BI-9307
Selectivity Number of Off-targets
0