Compound name
BI-3257
Protein family
HIV Capsid
Target name
HIV CA
Affinity biochemical (nM)
160
Affinity on-target cellular (nM)
70
CG-Set
Other targets set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0001728a
SMILES
CCCn1cc(-c2cc(C3C(=O)N(CC)c4ccc(C(F)(F)F)cc4N(c4ccccc4)C3=O)cc(OC)c2O)cn1
InChIKey
OWOFDBGWRLKZST-UHFFFAOYSA-N
Mode of action
Inhibitor
Negative control
BI-3545
Affinity biochemical definition
IC50
Affinity biochemical assay type
Capsid disassembly assay
Affinity Biochemical Source Knowledge
https://www.opnme.com/molecules/cai-inhibitor-bi-3257
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Antiviral replication assay (using C8166-LTR-luciferase cells infected with HIV-1 NL4-3 for 1.5 h)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/22496222/
Affinity on-target cellular relation
=
Selectivity platform
SafetyScreen44™ (Eurofins)
Selectivity platform number of targets
44
Selectivity remarks
Screened at 10 µM against 44 GPCRs, ion channels, and hydrolases, closest targets as % of control: 5HT2B (14%), full screening data available on OpenMe website, https://www.opnme.com/molecules/cai-inhibitor-bi-3257;
Selectivity Source Knowledge
https://www.opnme.com/molecules/cai-inhibitor-bi-3257
Selectivity Number of Off-targets
0