Compound name
NIK SMI1
Protein family
Protein Kinase
Target name
MAP3K14
Affinity biochemical (nM)
0.23
Affinity on-target cellular (nM)
34
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0001854a
SMILES
COc1cc(C(N)=O)nc(-c2cccc(C#C[C@]3(O)CCN(C)C3=O)c2)c1
InChIKey
LQSHXYHWYGKAMX-FQEVSTJZSA-N
NCBI gene ID
9020
UniProt ID
Q99558
Synonyms
NIK, HSNIK, FTDCR1B, HS
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
FP assay (using 10 µM ATP)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/29940120/
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Cellular NF-κB reporter assay (Dual Glo luciferase detection system, NFκB-LUC, Promega, HEK293 cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/29940120/
Affinity on-target cellular relation
=
Selectivity platform
Kinase panel (literature)
Selectivity platform number of targets
228
Selectivity remarks
Screened at 100 nM, closest targets as % of inhibition: NIK (99.7%), KHS1(77.2%), full screening data available in supporting information, compound 4f;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/29940120/
Selectivity Number of Off-targets
0