Compound name
(Z)-4-Hydroxytamoxifen
Protein family
Nuclear Receptors
Target name
NR3A2
Affinity on-target cellular (nM)
1
CG-Set
Nuclear Receptor set
Recommended Concentration
0.3 µM
Compound EUbOPEN ID
EUB0001891a
SMILES
CC/C(=C(\C1=CC=C(C=C1)O)/C2=CC=C(C=C2)OCCN(C)C)/C3=CC=CC=C3
InChIKey
TXUZVZSFRXZGTL-QPLCGJKRSA-N
Mode of action
Antagonist
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Reporter gene assay (U2OS cells were transfected with p(ERE)2-luc reporter and pSG5-ER? or pSG5-ER? expression plasmid) (NR3A1 and NR3A2), Gal4 reporter gene assay (CV-1 cells)
Affinity on-target cellular source knowledge
https://doi.org/10.1016/j.ejmech.2020.112191
https://doi.org/10.1016/j.bmc.2017.01.019
Affinity on-target cellular relation
0
Selectivity platform
Nuclear receptor panel, bromodomain and kinase panel, literature
Selectivity platform number of targets
23
Selectivity remarks
Screened at 0.3 µM against NR1A1, NR1B1, NR1C3, NR1F3, NR1H3, NR1I1, NR1I2, NR1I3, NR2A1, NR2B1, NR4A1, NR5A2 and VP16 in a Gal4 hybrid reporter gene assay (HEK293T cells were cotransfected with pFR-Luc reporter, pRL-SV40 control reporter and pFA-CMV containing the Gal4-DBD fused with human NR-LBD of interest or pECE-SV40-Gal4-VP16): clean selectivty profile with no significant agonism/antagonism detected; Screened at 20 µM in a bromodomain and kinase panel against ABL1, AURKA, BRD4, BRPF1, CDK2, CSNK1D, FGFR3, GSK3B, MAPK1, TRIM24 in DSF assays: clean selectivity profile with no significant binding (?Tm < 2 K); IC50 = 6500 nM (PLD1) and 20000 nM (PLD2) reported in literature
Selectivity Source Knowledge
https://doi.org/10.1021/cb500828m
Selectivity Number of Off-targets
2