Compound name
(Z)-4-Hydroxytamoxifen
Protein family
Nuclear Receptors
Target name
NR3B3
Affinity on-target cellular (nM)
600
CG-Set
Nuclear Receptor set
Recommended Concentration
3 µM
Compound EUbOPEN ID
EUB0001891a
SMILES
CC/C(=C(\C1=CC=C(C=C1)O)/C2=CC=C(C=C2)OCCN(C)C)/C3=CC=CC=C3
InChIKey
TXUZVZSFRXZGTL-QPLCGJKRSA-N
Mode of action
Inverse Agonist
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Reporter gene assay (U2OS cells were transfected with p(ERE)2-luc reporter and pSG5-ER? or pSG5-ER? expression plasmid) (NR3A1 and NR3A2), Gal4 reporter gene assay (CV-1 cells)
Affinity on-target cellular source knowledge
https://doi.org/10.1016/j.ejmech.2020.112191
https://doi.org/10.1016/j.bmc.2017.01.019
Affinity on-target cellular relation
0
Selectivity platform
Nuclear receptor panel, bromodomain and kinase panel, literature
Selectivity platform number of targets
23
Selectivity remarks
Screened at 3 µM against NR1A1, NR1B1, NR1C3, NR1F3, NR1H3, NR1I1, NR1I2, NR1I3, NR2A1, NR2B1, NR4A1, NR5A2 and VP16 in a Gal4 hybrid reporter gene assay (HEK293T cells were cotransfected with pFR-Luc reporter, pRL-SV40 control reporter and pFA-CMV containing the Gal4-DBD fused with human NR-LBD of interest or pECE-SV40-Gal4-VP16): clean selectivty profile with no significant agonism/antagonism detected; Screened at 20 µM in a bromodomain and kinase panel against ABL1, AURKA, BRD4, BRPF1, CDK2, CSNK1D, FGFR3, GSK3B, MAPK1, TRIM24 in DSF assays: clean selectivity profile with no significant binding (?Tm < 2 K); IC50 = 6500 nM (PLD1) and 20000 nM (PLD2) reported in literature
Selectivity Source Knowledge
https://doi.org/10.1021/cb500828m
Selectivity Number of Off-targets
2