Compound name
Y-27632
Protein family
Protein Kinase
Target name
PKN2
Affinity biochemical (nM)
70
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0002096a
SMILES
CC(C1CCC(CC1)C(=O)Nc1ccncc1)N
InChIKey
IYOZTVGMEWJPKR-UHFFFAOYSA-N
NCBI gene ID
5586
UniProt ID
Q16513
Synonyms
PRK2, Pak-2, STK7
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Cambrex PKLight ATP Detection assay (using luciferin–luciferase to quantify residual ATP)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/20462760/
Affinity biochemical relation
=
Selectivity platform
Kinase panel (Millipore)
Selectivity platform number of targets
222
Selectivity remarks
Screened at 1 µM, closest targets as % of residual activity: PKN2 (16%), ROCK2 (19%), PRKCH (26%), full screening data are available via Chembl and paper, compound was also screened at 10 µM, https://pubmed.ncbi.nlm.nih.gov/23398362/;
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(PRKCE) = 335 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(DMPK) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(CDC42) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(PRKCE) = 335 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(DMPK) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(CDC42) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/23398362/
Selectivity Number of Off-targets
1