Compound name
Y-27632
Protein family
Protein Kinase
Target name
ROCK1
Affinity biochemical (nM)
46
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0002096a
SMILES
CC(C1CCC(CC1)C(=O)Nc1ccncc1)N
InChIKey
IYOZTVGMEWJPKR-UHFFFAOYSA-N
NCBI gene ID
6093
UniProt ID
Q13464
Synonyms
p160ROCK
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Cambrex PKLight ATP Detection assay (using luciferin–luciferase to quantify residual ATP)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/20462760/
Affinity biochemical relation
=
Selectivity platform
Kinase panel (Millipore)
Selectivity platform number of targets
222
Selectivity remarks
Screened at 1 µM, closest targets as % of residual activity: PKN2 (16%), ROCK2 (19%), PRKCH (26%), full screening data are available via Chembl and paper, compound was also screened at 10 µM, https://pubmed.ncbi.nlm.nih.gov/23398362/;
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(PRKCE) = 335 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(DMPK) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(CDC42) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(PRKCE) = 335 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(DMPK) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
In-vitro potency (Cambrex PKLight ATP Detection assay, using luciferin–luciferase to quantify residual ATP): IC50(CDC42) >8000 nM, https://pubmed.ncbi.nlm.nih.gov/20462760/
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/23398362/
Selectivity Number of Off-targets
1