Compound name
SGC-GSK3-1
Protein family
Protein Kinase
Target name
GSK3A
Affinity biochemical (nM)
1
Affinity on-target cellular (nM)
4.6
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0002315a
SMILES
O=C(NCC1CCOCC1)c1n[nH]cc1NC(=O)c1c(F)cccc1F
InChIKey
OCQCKUTZHBQNEI-UHFFFAOYSA-N
NCBI gene ID
2931
UniProt ID
P49840
Mode of action
inhibitor
Negative control
SGC-CDKL5/GSK3-1N
Affinity biochemical definition
IC50
Affinity biochemical assay type
Enzymatic radiometric assays (Eurofins)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/37084253/
Affinity biochemical relation
0
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (HEK293T cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/37084253/
Affinity on-target cellular relation
0
Selectivity platform
DiscoverX (scanMAX)
Selectivity platform number of targets
403
Selectivity remarks
Screened at 1 µM, closest targets as % of control: GSK3A (0%), GSK3B (0.2%), DYRK1A (0.4%), ICK(5.7%), DYRK2 (8.3%), DYRK1B (10%), MAPK8 (16%), CDK4 (20%), RPS6KA4 (29%), CLK1 (30%), CDKL5 (30%), CDK7 (31%), MAPK10 (31%), CLK4 (32%), CLK2 (34%), CDKL2 (77%), CDKL1(93%), CDKL3(100%); In-vitro potency of closest targets (enzymatic radiometric assays, Eurofins): IC50(DYRK1A) = 170 nM, IC50(ICK) = 520 nM, IC50(DYRK2) = 800 nM, IC50(DYRK1B) = 47 nM, IC50(MAPK8) >10000 nM, IC50(CDK4) = 3800 nM, IC50(RPS6KA4) >10000 nM, IC50(CLK1) = 620 nM, IC50(CDKL5) = 840 nM, IC50(CDK7) >10000 nM, IC50(MAPK10) = 2900 nM, IC50(CLK4) = 760 nM, IC50(CLK2) = 800 nM; In-cellular potency (NanoBRET assay, HEK293T cells): IC50(DYRK1B) = 2400 nM, IC50(CDKL5) = 1400 nM
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/37084253/
Selectivity Number of Off-targets
8