Compound name
TP-060
Protein family
Transferase
Target name
UGCG
Affinity biochemical (nM)
31
Affinity on-target cellular (nM)
7.6
CG-Set
Other targets set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0002336a
SMILES
CC(C)(O)c1ccc(N2Cc3c(ccnc3-c3ccc(F)cc3OCC(F)(F)F)C2=O)cc1
InChIKey
OAZAPIKYVGRNCO-UHFFFAOYSA-N
NCBI gene ID
7357
UniProt ID
Q16739
Synonyms
GCS, GLCT1
Mode of action
Allosteric inhibitor
Negative control
TP-060n
Affinity biochemical definition
IC50
Affinity biochemical assay type
Enzymatic assay
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/35188773/
Affinity biochemical relation
=
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Glucosylceramide lowering assay (using GBA mutant human fibroblast cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/35188773/
Affinity on-target cellular relation
=
Selectivity platform
SAFETYscan (DiscoverX)
Selectivity platform number of targets
47
Selectivity remarks
Screened against a panel of receptors, ion channels, and other enzymes (enzymatic assays), in-vitro potency of closest target: IC50(SLC6A4) = 0.31 µM, IC50(HTR1B) = 2.8 µM, IC50(CHRNA4) = 6.5 µM, other targets IC50 >10 µM, full screening data available as supplement information;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/35188773/
Selectivity Number of Off-targets
SLC6A4