Compound name
TR03307825
Protein family
Protein Kinase
Target name
EGFR
Affinity biochemical (nM)
6
Affinity on-target cellular (nM)
120
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0002414a
SMILES
Clc1cc(ccc1)Nc2ncnc3c2cc(c(c3)OC)OC
InChIKey
GFNNBHLJANVSQV-UHFFFAOYSA-N
NCBI gene ID
1956
UniProt ID
P00533
Synonyms
ERBB1
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Radiometric assay (conc. ATP = 0.4 µM)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/10090785/
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
ELISA (inhibition of EGF-dependent tyrosine phosphorylation, using A431 cells)
Affinity on-target cellular relation
=
Selectivity platform
Kinase panel (Millipore)
Selectivity platform number of targets
222
Selectivity remarks
Screened at 1 µM, closest targets as % of residual activity: EGFR (-5%), LYN(10%), BRSK1 (14%), full screening data are available at Chembl and in paper, also screened at 10 µM, https://pubmed.ncbi.nlm.nih.gov/23398362/;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(ABL1) = 125 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(SRC) >10000 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(PRKCA) >10000 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (ADP Hunter assay, conc. ATP = 100 µM): IC50(MNK1) = 530 nM, https://pubs.acs.org/doi/10.1021/jm1005513#_i23;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(ABL1) = 125 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(SRC) >10000 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (radiometric assay, conc. ATP = 0.4 µM): IC50(PRKCA) >10000 nM, https://pubmed.ncbi.nlm.nih.gov/10090785/;
In-vitro potency (ADP Hunter assay, conc. ATP = 100 µM): IC50(MNK1) = 530 nM, https://pubs.acs.org/doi/10.1021/jm1005513#_i23;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/23398362/
Selectivity Number of Off-targets
1