Compound name
MS184
Protein family
Nuclear Receptors
Target name
NR4A3
Affinity biochemical (nM)
170
Affinity on-target cellular (nM)
110
CG-Set
Nuclear Receptor set
Recommended Concentration
0,3 µM
Compound EUbOPEN ID
EUB0003338a
SMILES
ClC1=CC=CN2C1=NC(C)=C2NC(C3=CC=C(C4=CC=C(N(C)C)C=C4)O3)=O
InChIKey
HGXSOLYLDMLDRI-UHFFFAOYSA-N
NCBI gene ID
8013
UniProt ID
Q92570
Synonyms
CSMF, CHN, NOR1, MINOR
Mode of action
Agonist
Affinity biochemical definition
Kd
Affinity biochemical assay type
ITC (NR4A2)
Affinity Biochemical Source Knowledge
https://doi.org/10.1038/s42004-024-01224-0
Affinity on-target cellular definition
EC50
Affinity on-target cellular assay type
Gal4 hybrid reporter gene assay (HEK293T cells were cotransfected with pFR-Luc reporter, pRL-SV40 control reporter and pFA-CMV-hNur77-LBD or pFA-CMV-hNurr1-LBD, or pFA-CMV-hNOR1-LBD expression plasmid)
Affinity on-target cellular source knowledge
https://doi.org/10.1038/s42004-024-01224-0
Affinity on-target cellular relation
0
Selectivity remarks
Screened at 0.3 µM and 3 µM against NR1A1, NR1B1, NR1C1, NR1C2, NR1C3, NR1F3, NR1H3, NR1H4, NR1I1, NR1I3, NR2A1, NR2B1, NR5A2 and VP16 in a Gal4 hybrid reporter gene assay (HEK293T cells were cotransfected with pFR-Luc reporter, pRL-SV40 control reporter and pFA-CMV containing the Gal4-DBD fused with human NR-LBD of interest or pECE-SV40-Gal4-VP16): clean selectivty profile with no significant agonism/antagonism
Selectivity Source Knowledge
https://doi.org/10.1038/s42004-024-01224-0