Compound name
FMF-04-159-2
Protein family
Protein Kinase
Target name
CDK2
Affinity biochemical (nM)
8.2
Affinity on-target cellular (nM)
256
CG-Set
Kinase set
Recommended Concentration
1 µM
Compound EUbOPEN ID
EUB0000057b
SMILES
CN(C)C/C=C/C(=O)Nc1cccc(S(=O)(=O)N2CCC(NC(=O)c3n[nH]cc3NC(=O)c3c(Cl)cc(Cl)cc3Cl)CC2)c1
InChIKey
HZPYSAHDRSBARR-QPJJXVBHSA-N
NCBI gene ID
1017
UniProt ID
P24941
Mode of action
Covalent inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Z'LYTE kinase assays (using CDK2/CycA, conc. ATP = Km)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/30930164/
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
NanoBRET assay (using CDK2/cylinY, HCT116 cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/30930164/
Affinity on-target cellular relation
=
Selectivity platform
KiNativ profiling (using HCT116 cells)
Selectivity platform number of targets
289
Selectivity remarks
Screened at 1 µM, 4h incubation, closest target as % of inhibition: CDK17 (94.2%), CDK18 (8.%), full screening data are available as supporting info,https://pubmed.ncbi.nlm.nih.gov/30930164/;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/30930164/
Selectivity Number of Off-targets
1