Chemical probes are cell-active, selective, highly validated research tools that can be used to decipher the biology of their target. They are useful assets for example, in phenotypic assays, or as a starting point for medicinal chemistry campaigns. Chemical probes developed by the EUbOPEN consortium are peer reviewed by an external committee and available upon request. The probes are released with a structurally similar inactive negative control compound (where feasible) and fulfill the following criteria:
| Potency | ≤ 100 nM in a biochemical or biophysical assay |
| Selectivity |
≥ 30-fold over related proteins |
| Evidence of target engagement in cells | ≤ 1 µM for the druggable target or 10 µM for shallow PPI targets |
| Cytotoxicity | ≥ 10 µM, unless target mediated |
For more information, or to request a probe, click on the probe link below.
| Probe name | Target | Negative control | Web link | Probe package |
|---|---|---|---|---|
| SGC-PI5P4Kγ/MYLK4-1 | PI5P4Kγ and MYLK4 | SGC-PI5P4Kγ/MYLK4-1N | SGC-PI5P4Kγ/MYLK4-1: A Chemical Probe for PI5P4Kγ and MYLK4 | EUbOPEN_ProbeSheet_PI5P4Kgamma_v1.pdf193.67 KB |
| SGC-CK2-2 | CK2, CSNK2 | SGC-CK2-2N | SGC-CK2-2: A Chemical Probe for CK2 | EUbOPEN_ProbeSheet_CK2_2_v1.pdf226.18 KB |
| BAY-805 | USP21 (ubiquitin-specific-processing protease 21) | BAY-728 | BAY-805: A chemical probe for USP21 | EUbOPEN_ProbeSheet_BAY-805_v1.pdf174.79 KB |
| FHT-2344 | SMARCA2/ SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2/4) | FHT-5908 | FHT-2344: A Chemical Probe for SMARCA2/4 | EUbOPEN_ProbeSheet_FHT_2344_v1.pdf188.78 KB |
| BI-7190 | BPTF (Bromodomain PHD Finger Transcription Factor) | BI-4827 | BI-7190: A Chemical Probe for BPTF | EUbOPEN_ProbeSheet_BI-7190_v1-1.pdf205.49 KB |
| CS640 | CAMK1D | CS640n | CS640: A Chemical Probe for CAMK1D | EUbOPEN_ProbeSheet_CAMK1D_v1.pdf215.75 KB |
| MRK-990 | PRMT9/PRMT5 | MRK-990-NC | MRK-990: A chemical probe for PRMT9/5 | EUbOPEN_ProbeSheet_MRK-990_v2.pdf200.06 KB |
| MRK-952 | NUDT5 | MRK-952NC | MRK-952: A chemical probe for NUDT5 | EUbOPEN_ProbeSheet_MRK-952_v1.pdf168.01 KB |
| MU1742 | CK1δ, CK1ε, CSNK1δ, CSNK1ε, Casein kinase 1 delta, Casein kinase 1 epsilon | MU2027 | MU1742: A Chemical probe for CK1δ and CK1ε | EUbOPEN_ProbeSheet_MU1742_v1.pdf200.32 KB |
| SGC-PIKFYVE-1 | PIKfyve | SGC-PIKFYVE-1N | SGC-PIKFYVE-1: A Chemical Probe for PIKfyve | EUbOPEN_ProbeSheet_PIKfyve_v1.pdf210.6 KB |
| BAY 1816032 | BUB1 Mitotic Checkpoint Serine/Threonine Kinase, HBUB1 | BAY-283 | BAY 1816032: A Chemical Probe for BUB1 | EUbOPEN_ProbeSheet_BAY 1816032 v1.pdf222.97 KB |
| JNJ-9350 | SMOX (spermine oxidase) | JNJ-4545 | JNJ-9350: A Chemical Probe for SMOX | EUbOPEN_ProbeSheet_JNJ_9350_v1.pdf209.72 KB |
| JNJ-6204 | CSNK1D (Casein Kinase 1 Delta, CKID), CSNK1E (Casein Kinase 1 Epsilon, CKIE) | JNJ-0293 | JNJ-6204: Dual Chemical Probe for CSNK1D, CSNK1E | EUbOPEN_ProbeSheet_JNJ-6204_v1.pdf205.15 KB |
| JNJ-39758979 | HRH4 (Histamine Receptor H4, GPCR105, H4R) | JNJ- 39668551 | JNJ-39758979: A Chemical Probe for HRH4 | EUbOPEN_ProbeSheet_JNJ-39758979_v1.pdf149.23 KB |
| CCT369260 | BCL6 (BCL6 transcription repressor, Zinc Finger Protein 51) | CCT393732 | CCT369260: A Chemical Probe for BCL6 | EUbOPEN_ProbeSheet_CCT369260_v1.pdf171.23 KB |
| SAFit2 | FKBP5 (FKBP prolyl isomerase 5, FKBP51) | ddSAFit2 | SAFit2: A Chemical Probe for FKBP5 | EUbOPEN_ProbeSheet_SAFit2_v1.pdf169.59 KB |
| TP-051 | FFAR1 (free fatty acid receptor 1), GPR40 | TP-051n | TP-051: A Chemical Probe for FFAR1 | EUbOPEN_ProbeSheet_TP-051_v1.pdf883.13 KB |
| TP-050 | GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), GluN2A | TP-050n | TP-050: A Chemical Probe for GRIN2A | EUbOPEN_ProbeSheet_TP_050_v1.pdf884.11 KB |
| BI-2081 | FFAR1 (free fatty acid receptor 1), GPR40 | BI-0340 | BI-2081: A Chemical Probe for FFAR1 | EUbOPEN_ProbeSheet_BI_2081_v1.pdf895.11 KB |
| MU1700 | ALK1, ALK2 | MU1700NC | MU1700: A Chemical Probe for ALK1/2 | EUbOPEN_Probesheet_ALK1+2_MU1700.pdf657.46 KB |